1. To evaluate the efficiency and profitability of microalgae (preferably marine) cultivated in advanced tPBR (new designs for vertical tubular photobioreactors) as applied to:
a. The production of biodiesel from the algal biomass produced (in the form of a concentrated microalgal ‚Äúpaste‚ÄĚ (85-90% water) with a dry weight TAGcontent of 25-52%).
b. Photosynthetic sequestration of industrial CO2
2. Isolate, identify (conventional and molecular) and preserve (conventional and cryopreservation) microalgae and cyanobacteria (preferably marine) from tropical and subtropical areas that exhibit any of the following characteristics: high hydrocarbon or lipid (triglycerides) content and or exhibiting or containing acetyl-CoA carboxylase genes or enzymes, large in size and resistant to high temperatures, CO2 and shear forces.
3. Selection of the species and strains described in item 2 that show elevated growth rates in vertical tubular photobioreactors (tPBR).
4. Cultivate select species in conventional tPBR.
5. Evaluate the conversion of concentrated microalgal biomass (10-15% ps) with a high water content (85-90%) into biodiesel.
6. Develop advanced tPBR with improved output per unit volume (in comparison to a conventional tPBR) by increasing light capture and photosynthetic efficiency (by introducing the following variables: vortex agitation, light pulsing by microturbulence, solar thermoregulation, nighttime irradiation, integration of six PBR into a single cultivation unit, fibre optic light injection).
7. Develop a PBR automatic control program.